Coding

Part:BBa_K678041:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)


pyrG-DR cassette, for selection of Aspergillus nidulans


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 331
    Illegal XbaI site found at 362
    Illegal PstI site found at 1169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 331
    Illegal NheI site found at 1825
    Illegal PstI site found at 1169
    Illegal NotI site found at 1816
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 331
    Illegal BglII site found at 2138
    Illegal BamHI site found at 356
    Illegal BamHI site found at 907
    Illegal XhoI site found at 767
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 331
    Illegal XbaI site found at 362
    Illegal PstI site found at 1169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 331
    Illegal XbaI site found at 362
    Illegal PstI site found at 1169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 960
    Illegal SapI.rc site found at 98
    Illegal SapI.rc site found at 1909


Design Notes

Compatible with the Plug'n'Play assembly standard


Source

pDel2

References